Description
Description: This assay employs a two-site sandwich ELISA to quantitate IL17RC in samples. An antibody specific for IL17RC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL17RC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL17RC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL17RC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Interleukin-17 receptor C is a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date.The predicted full-length 720-amino acid protein has an N-terminal signal peptide, followed by an acidic extracellular domain with 9 potential N-glycosylation sites, a single-pass transmembrane domain, and a basic cytoplasmic region with multiple phosphorylation sites. It has a calculated molecular mass of 76.4 kD and shares 22% amino acid identity with IL17R.strongest expression in prostate, kidney, liver, heart, and muscle, with barely detectable levels in thymus and peripheral blood leukocytes